Hudson-robotics RapidWash Manuel d'utilisateur

RapidWash User Manual Rev 1.2 10 Stern Avenue, Springfield, NJ 07081 Tel: 973-376-7400 ♦ Fax: 973-376-8265

10 Figure 11 Dispense – Select the liquid number (1 or 2) and the desired volume, in uL (Figure 12). Note: If the liquid (1 o

11 Figure 13 Example of Wash Program (Figure 14) Figure 14 All program values and steps are automatically saved to that program, as they appe

12 2.3 - Prime The Prime feature allows you to select the liquid number and the total volume (in uL) dispensed through the dispense manifold. Pri

132.4 - Auto Prime Auto Prime performs exactly like the Prime feature but will run automatically at the user determined interval (Figure 16) Volu

142.5 - Maintenance Mode The features in this section are used to clean and maintain the performance of the dispense and aspirate manifolds (Figure

15 Figure 18 Plate Height - Operates as described previously Cycles - The number of times the cleaning process will be performed Volume - This is

16by the number of wells (i.e. a volume of 9600 would lead to 9.6 mL dispensed across a 96 well plate or 100 uL in every well). If run without the

172.6 – Change Settings This Section allows you to modify all the performance settings of the RapidWash (Figure 19) Figure 19 Settings Tab: As

18 Initialize on Powerup- This will allow the RapidWash to automatically home when the unit is turned on. The default setting is auto initialize

19 Figure 21 Aspirate Origin- This is the saved position of the aspirate manifold when the RapidWash begins to aspirate. This position is calibr

2Index Section 1 - Setup ... 3 1.1- T

20Dispense Origin- This is the saved position of the dispense manifold when the RapidWash begins to dispense. This position is calibrated with a 96

21 Figure 25 Plate Origin- This is the starting position (origin) of the aspirate tips relative to the teaching plate. To teach this position move

22Calibration tab: The validation setting turn on/off various checks that the unit can perform prior to executing programs and priming. Validatio

23Section 3 - Maintenance of the RapidWash Manifold 3.1 - Removal and Installation of the Manifold The RapidWash is shipped without the manif

24 Figure 28 Slide the manifold onto the locating pins and continue to slide the manifold towards the mount until it locks into place (Figure 29).

253.2 - Disassembly of the Manifold Remove the flat head screws that hold the aspirate and dispense manifolds together. If desired, the standoffs m

26Section 4 - Hardware Specifications Section 4.1 - General NOTE: Specifications are subject to change without notice. RapidWash: • Plate

27Manifold: • Manifold Material: Acetal Copolymer • Sealing O-rings Viton • Fixed tip Material: Stainless Steel Section 4.3 - Dimens

3Section 1 - Setup 1.1- Tubing and Connections Figure 1 1- Touch Screen Display Connection 2- RS-232 Communication Connection 3- AC Inlet F

4 Figure 2 Figure 3 1.2 - Bottles Liquid 1 Liquid 1 and 2 Dispense Bottle Liquid Level Sensor location Tubing to Liquid 1 or Liquid 2 Port on

5• Pressure In- From 12psi pressure source (#9 from figure 1) from the rear of the RapidWash to top of bottle (1/4” blue tubing-See Figure 2) • L

6 Figure 4 Figure 5 RapidWash Select Manifold

7Section 2 - Operation of RapidWash through the Touch Screen Controller 2.1 - Key Pad Functions Wash – Up to 20 Wash programs can be created and s

82.2 - Wash The RapidWash can support up to 20 dispense programs. Press the Next or Prev key (Figure 8) on the Touch Panel to select the program n

9 Figure 9 Figure 10 Aspirate – Allows selection of: Depth - The distance in mm traveled down into the plate wells, measured