Hudson-robotics RapidPick Manuel d'utilisateur Page 1

1 RapidPick Colony Picking and Rearraying User Manual 10 Stern Avenue, Springfield, NJ 07081 Tel: 973-376-7400  Fax: 973-376-8265

10 B. View Rearray Logs Similar tabular data is available from rearraying operations that have been run: The Source Plate ID and wells are shown

11 Section 2 – Developing and Editing Protocols 2.1 – Protocol Editor SoftLinx V Protocol Editor allows the user to create a

12 2.2 – Protocol Template Each SoftLinx RapidPick Protocol is built using the same template and has 3 sections: - Initial Loading of Plates - Ma

13 2.3 – RapidPick SoftLinx Steps The RapidPick interface has multiple steps that can be used in a SoftLinx protocol. Each one is shown in detail

14 Limit number of colonies to pick: Maximum number of colonies to pick within this scan. Pad Wells: Reserve the maximum number of wells that coloni

15 2.4 - Picking Segments The Pick Colonies and Segmented Picking commands allow users to focus the picking commands onto specific areas of a plate.

16 2.5 – Common Issues The Picker software does not connect to the instrument or show an “Online” status. - Ensure the picker is on before the soft

17 The picker seems to be picking colonies accurately, but colonies are having issues growing. - Ensure that all target plates are sterile. Do manua

2 Index Introduction ... 3 Sec

3 Introduction Hudson's RapidPick workcell is a customizable, automated colony picking and rearraying solution fit for any users’ needs. The R

4 Section 1 – Workcell Operation 1.1 – Startup All instruments must be powered and connected to the computer prior to starting the software packages

5 The selected protocol will be loaded and shown in the main SoftLinx screen: At this point, the user can load all plates into the prope

6 1.2 – Starting a Run – Checklist The following items should be checked prior to starting a SoftLinx RapidPick Protocol. This list assumes all ca

7 1.3 – Protocol Execution Protocols are used to run the full system. Protocols generally follow the same template of load plates, run the picker, an

8 1.4 – Log Data The RapidPick logs all colonies that have been picked, including image of plates, and saves them in multiple formats for the user to

9 A. View Picked Colony Data This selection allows the user to view Source/Destination relationships between each actual colony picked and the targe